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BBa_J22005

BBa_J22005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J22005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ruchi Malik

Date created: 2006-09-03 11:00:00

Date modified: 2015-08-31 04:08:38

Tet promoter-Tsr receptor-RFP Reporter

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_J22005_sequence (Version 1)

Description

This part contains Tsr chemotaxis receptor under transcriptional control of Tetracycline promoter. Transcriptional activity is reported by RFP reporter downstream of Tsr gene.

Notes

Source

Tsr coding sequence was amplified using PCR from Muk21 E.coli strain.
Rest of the sequences were taken from the registry parts.

Components

Access	Instance	Definition
public public public public public public public	BBa_R0040 BBa_B0032 BBa_C0620 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012	p(tetR) BBa_B0032 BBa_C0620 BBa_B0034 eyfp BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0032 BBa_C0620 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012	1,54 63,75 82,1737 1746,1757 1764,2486 2495,2574 2583,2623	p(tetR) BBa_B0032 BBa_C0620 BBa_B0034 eyfp BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J22005/1