BBa_J23018

BBa_J23018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-08-02 11:00:00
Date modified: 2015-08-31 04:08:39

EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser"



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_J23018_sequence (Version 1)

Description

Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.

Notes

N/A

Source

J61003 and E0040

Sequence Annotation Location Component / Role(s)
BBa_B0015 terminator
BBa_R0040 (Ptet)
pBR322 origin
pMB1 origin
beta-lactamase (ampicillin resistance marker)
beta-lactamase (ampR) promoter
rrnB T1 terminator
BioBrick suffix
EcoRI
NotI
XbaI
Verification forward (VF2) primer binding site
Verification reverse (VR) primer binding site
BBa_B0034
RBS
Ptet
2080,2208
2217,2270
183,802
180,895
957,1817
1859,1887
2091,2134
1,21
2058,2063
2064,2071
2292,2297
1940,1959
79,98
2279,2290
2279,2290
2217,2270
engineered_region feature/BioBrick
feature/BioBrick engineered_region
sequence_feature feature/dna
sequence_feature feature/dna
CDS feature/cds
promoter feature/promoter
stem_loop feature/stem_loop
sequence_feature feature/dna
sequence_feature feature/dna
sequence_feature feature/dna
feature/dna sequence_feature
feature/primer_binding primer_binding_site
feature/primer_binding primer_binding_site
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
feature/promoter promoter
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J23018/1