Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Liang
Date created: 2006-08-24 11:00:00
Date modified: 2015-08-31 04:08:39
[RA2 Promoter][RBS][trbCf][TT]
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_J23075_sequence (Version 1) |
Description
[RA2 Promoter][RBS][trbCf][TT]Notes
pSB1A2-J23038 has a really short sequence between XbaI and SpeI so okay to PCR because miniprep was messy.Source
[RA2 Promoter][RBS][trbCf][TT]Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )
Paste into pSB1AK3-J23028 (XbaI/BsaI, Neb2, Large 5229)
Product is pSB1AK3-J23075.
| Access | Instance | Definition |
| public public public public public | BBa_J23104 BBa_B0034 BBa_J23014 BBa_B0010 BBa_B0012 | BBa_J23104 BBa_B0034 [trbCf] BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23104 BBa_B0034 BBa_J23014 BBa_B0010 BBa_B0012 | 1,35 44,55 62,703 712,791 800,840 | BBa_J23104 BBa_B0034 [trbCf] BBa_B0010 BBa_B0012 |