BBa_J24014

BBa_J24014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J24014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jamie Lemon

Date created: 2006-06-29 11:00:00

Date modified: 2015-08-31 04:08:41

Toggle Switch to Immobilize E. Coli

Details

• Types: DnaRegion
• Roles: engineered_region; Project
• Sequences: BBa_J24014_sequence (Version 1)

Description

This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine.

Notes

Still planning.

Source

agg

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0033	BBa_B0033
public	BBa_C0078	lasI
public	BBa_S03271	MotB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0033	209,219	BBa_B0033
BBa_C0078	226,892	lasI
BBa_S03271	901,1837	MotB

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J24014/1

Member of these Collections

• iGEM Parts Registry