Types | DnaRegion
|
Roles | engineered_region
Composite
|
Sequences | BBa_J36838_sequence (Version 1)
|
Description
This part is an assembly of the lipoprotein 20aa signal peptide and its 1st nine amino acids, with amino acids 46-66 of outer membrane protein A. When fused with a protein downstream, this surface display vehicle should express a protein on the outer surface of E. coli.
Notes
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Source
Assembly of BBa_J36835 and BBa_J36836