Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Perry Tsai
Date created: 2006-10-30 12:00:00
Date modified: 2015-08-31 04:08:47
Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_J36847_sequence (Version 1) |
Description
Assembled construct of signal peptide of lipoprotein, five transmembranes domain of outer membrane protein A, and streptavidin, single-chain dimeric with His6 tag at the end.This construct codes for a protein which displays streptavidin on the cell surface.
Notes
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.
Source
Assembly of J36835, J36837, J36843| Access | Instance | Definition |
| public public public | BBa_J36835 BBa_J36837 BBa_J36843 | BBa_J36835 BBa_J36837 BBa_J36843 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J36835 BBa_J36837 BBa_J36843 | 1,87 96,437 446,1264 | BBa_J36835 BBa_J36837 BBa_J36843 |