BBa_J44012

BBa_J44012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J44012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Todd Eckdahl

Date created: 2006-10-25 11:00:00

Date modified: 2015-08-31 04:08:49

HixC-pBAD-HixC-RBS-TetF-HixC

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_J44012_sequence (Version 1)

Description

Construct for use in detecting the activity of Hin recombinase system. pBAD promoter is flanked by HixC recombination sites and may be flipped from the right to the left orientation. Tet resistance gene with upsteam RBS are also flanked by HixC sites and may be flipped from forward to reverse orientation. Cloned in pSB1A7 - readthrough transcription not detected.

Notes

Trascription readthrough into part appears to have been blocked by the introduction of double forward terminators before and after the cloning site.

Source

Cloned from BBa_J44010

Components

Access	Instance	Definition
public	BBa_J44000	hixC
public	BBa_I13453	BBa_I13453
public	BBa_J44000	hixC
public	BBa_B0030	BBa_B0030
public	BBa_J31007	tetA(C)f
public	BBa_J44000	hixC

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J44000	1,26	hixC
BBa_I13453	35,164	BBa_I13453
BBa_J44000	173,198	hixC
BBa_B0030	207,221	BBa_B0030
BBa_J31007	228,1418	tetA(C)f
BBa_J44000	1427,1452	hixC

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J44012/1

Member of these Collections

• //function/recombination/hin
• iGEM Parts Registry