Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yusaku Nakashima
Date created: 2006-10-20 11:00:00
Date modified: 2015-08-31 03:48:12
LacITandem_regulated(B-5-2)
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_J54252_sequence (Version 1) |
Description
First BglII site was deleted.Notes
LacI regulatedSource
synthesis| Sequence Annotation | Location | Component / Role(s) |
| Sal BBa_J54023 BBa_J54200 BamHI lacq Promoter BBa_J54250 LacI_Binding_Site BBa_J54250 LacI_Binding_Site BglII BBa_J54034 MulI RBS-1\Strong BBa_B0030 RBS BBa_E0040 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,5 1,5 14,63 20,26 27,61 72,113 77,100 122,163 127,150 151,156 172,176 172,176 185,199 185,199 192,196 206,925 934,1013 945,988 1022,1062 1029,1048 1049,1062 1055,1055 | feature/tag tag engineered_region feature/BioBrick feature/BioBrick engineered_region feature/tag tag promoter feature/promoter feature/BioBrick engineered_region feature/operator operator engineered_region feature/BioBrick operator feature/operator feature/tag tag engineered_region feature/BioBrick feature/tag tag ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/BioBrick engineered_region stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon |