Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Charles Yoon
Date created: 2006-10-30 12:00:00
Date modified: 2015-08-31 03:22:59
Cell-See-Us Bacterial Thermometer
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_J5530_sequence (Version 1) |
Description
The bacteria will produce different ratios of GFP and mRFP in response to temperature sensitive LacI (LacI ts). At high temperatures, mostly mRFP will be produced and will fluoresce red. As the temperature decreases, more and more GFP is produced relative to mRFP so the overall fluorescence will shift to green.Notes
Addition of external control factors such as IPTG and aTc to modulate GFP and mRFP signal strengths.Source
iGEM 2006 Registry| Access | Instance | Definition |
| public public public public public public | BBa_I0500 BBa_J06801 BBa_Q04400 BBa_I13504 BBa_R0011 BBa_I13507 | pBad/araC BBa_J06801 tetR BBa_I13504 lacI+pL BBa_I13507 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_J06801 BBa_Q04400 BBa_I13504 BBa_R0011 BBa_I13507 | 1,1210 1219,2589 2598,3499 3508,4382 4391,4444 4454,5314 | pBad/araC BBa_J06801 tetR BBa_I13504 lacI+pL BBa_I13507 |