Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Valencia iGEM 2006
Date created: 2006-10-25 11:00:00
Date modified: 2015-08-31 03:14:48
Promoter activated synergistically by cI and CRP with the reporter RFP
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J58101_sequence (Version 1) |
Description
A promoter activated synergistically by cI and CRP proteins (part ref. BBa_J58011) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.Notes
Notice that in the promoter sequence the XbaI restricction site is present. Therefore, we have to digest this part upstream only with EcoRI.Source
To make this composite part we assemby two parts from the Registry (a promoter and a generator).| Access | Instance | Definition |
| public public | BBa_J58100 BBa_I13507 | pAND BBa_I13507 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J58100 BBa_I13507 | 1,106 115,975 | pAND BBa_I13507 |