| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_J58102_sequence (Version 1)
|
Description
Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated negatively (repressed) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors.
Notes
none
Source
To make this composite part we assemby two parts from the Registry (a promoter (R0082) and a generator (I13504)).
