Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-09-20 11:00:00
Date modified: 2015-08-31 02:02:59
[FRT][R6K][CmR][FRT]
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_J61027_sequence (Version 1) |
Description
Cassette for knockins or knockouts with chloramphenicol selection and a silent origin of replication. The cassettes can be removed by introduction of flp recombinase expressing helper plasmid pCP20 leaving behind a single FRT site scar. Digestion of the genomic DNA containing these cassettes followed by ligation and transformation into a pir116 or pir+ strain allows capture of the cassette as a high or medium-copy plasmid.pSB1A2-Bca9008
Notes
N/ASource
standard biobrick assembly| Access | Instance | Definition |
| public public public public | BBa_J61020 BBa_J61001 BBa_J61000 BBa_J61020 | FRT R6K CmR FRT |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J61020 BBa_J61001 BBa_J61000 BBa_J61020 | 1,34 43,448 457,1350 1359,1392 | FRT R6K CmR FRT |