TNY2AKUB

BBa_K076000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K076000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM2008 Princeton team

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:08:32

pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K076000_sequence (Version 1)

Description

A plasmid allowing rtTA-induced expression of the cell-fate regulator Ngn1 linked to an enhanced yellow fluorescent protein reporter, and a red fluorescent protein reporter tethered by a 2A linker. This construct allows for the verification of the 2A linker, with the ideal result of yellow-labeled Ngn1 remaining in the nucleus and red-labeled mKate being exported to the cytoplasm.

Notes

Constructed by PCR SOEing.

Source

This plasmid was constructed by digesting pLV-TRE-Sox17-Ubc-Bla with SfiI to excise TRE-Sox17, and inserting the PCR SOEd SfiI-Ngn1-linker-EYFP-2A-mKate-SfiI.

Components

Access	Instance	Definition
public	BBa_K076001	BBa_K076001
public	BBa_K076002	BBa_K076002
public	BBa_K076003	BBa_K076003
public	BBa_K076004	BBa_K076004
public	BBa_K076005	BBa_K076005
public	BBa_K076006	BBa_K076006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K076001	1,10859	BBa_K076001
BBa_K076002	10866,11597	BBa_K076002
BBa_K076003	11606,11659	BBa_K076003
BBa_K076004	11666,12382	BBa_K076004
BBa_K076005	12391,12456	BBa_K076005
BBa_K076006	12463,13176	BBa_K076006

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K076000/1

Member of these Collections

• iGEM Parts Registry