Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francesca Ceroni
Date created: 2008-10-23 11:00:00
Date modified: 2015-05-08 01:08:34
GFP expression controlled by LacI repressor under the BBa_J23114 promoter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K079029_sequence (Version 1) |
Description
...Notes
We wanted to characterize the Lac promoter repression by different levels of LacI protein. Thus, we cloned the BBa_S0100 part under the control of three promoters from the Berkley costitutive promoter librarywith different transcriptional strenght: BBa_J23114, BBa_J23105and BBa_J23118. As a reporter, the GFP protein (BBA_I763020) was cloned downstream of the Lac promoter (BBa_J04500).Source
Registry standard parts| Access | Instance | Definition |
| public public public public public | BBa_J23114 BBa_S0100 BBa_B0015 BBa_R0010 BBa_I763020 | BBa_J23114 BBa_S0100 BBa_B0015 LacI RBS-GFP-Te |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23114 BBa_S0100 BBa_B0015 BBa_R0010 BBa_I763020 | 1,35 44,1214 1223,1351 1360,1559 1568,2481 | BBa_J23114 BBa_S0100 BBa_B0015 LacI RBS-GFP-Te |