BBa_K081024

BBa_K081024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K081024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lorenzo Pasotti, Paolo Magni
Date created: 2008-10-18 11:00:00
Date modified: 2015-05-08 01:08:35

pcyA protein generator and Pomp



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K081024_sequence (Version 1)

    Description

    This device is a PoPS in -> pcyA out with Pomp promoter downstream.

    Pomp (BBa_R0082) is a positively regulated, OmpR-controlled promoter. Phosphorylated OmpR binds to the three operator sites and activates transcription.

    Proteins pcyA, ho1 and cph8 can produce a photosensitive complex that can activate the endogenous transcription factor OmpR. So, light (in particular 660 nm radiation) is an indirect repressor of Pomp.

    These interactions are valid if used in E.coli deficient in natural EnvZ, because EnvZ can activate OmpR.
    {|align="center"
    [[Image:pv_K081024.png|400px]]
    |}

    Notes

    We used BioBrick Standard Assembly.

    Source

    RBS:
    pcyA:
    T:
    Pomp:

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K081024/1
     

    Attachments

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