BBa_K091150

BBa_K091150 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091150

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Feeney

Date created: 2008-07-17 11:00:00

Date modified: 2015-05-08 01:08:38

pLas' Testing Construct C

Details

• Types: DnaRegion
• Roles: engineered_region; Signalling
• Sequences: BBa_K091150_sequence (Version 1)

Description

This part contains a constitutively active promoter, rbs, LasR coding region, and LuxR coding region followed by the pLas' promoter and GFP. K091150 generates both LasR and LuxR to test crosstalk and competitive binding with the pLas' promoter in the presence of AI-1 and PAI-1.

Notes

pLas' Testing Construct C has the coding region for luxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct D.

Source

The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.

Components

Access	Instance	Definition
public	BBa_S03982	BBa_S03982
public	BBa_S03986	BBa_S03986

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_S03982	1,842	BBa_S03982
BBa_S03986	851,2804	BBa_S03986

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091150/1

Member of these Collections

• iGEM Parts Registry