Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erin Feeney
Date created: 2008-07-17 11:00:00
Date modified: 2015-05-08 01:08:38
pLas' Testing Construct C
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K091150_sequence (Version 1) |
Description
This part contains a constitutively active promoter, rbs, LasR coding region, and LuxR coding region followed by the pLas' promoter and GFP. K091150 generates both LasR and LuxR to test crosstalk and competitive binding with the pLas' promoter in the presence of AI-1 and PAI-1.Notes
pLas' Testing Construct C has the coding region for luxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct D.Source
The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.| Access | Instance | Definition |
| public public | BBa_S03982 BBa_S03986 | BBa_S03982 BBa_S03986 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_S03982 BBa_S03986 | 1,842 851,2804 | BBa_S03982 BBa_S03986 |