BBa_K091165

BBa_K091165 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091165

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Gordon

Date created: 2008-07-28 11:00:00

Date modified: 2015-05-08 01:08:38

pLux/cI+RBS+GFP+TT

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K091165_sequence (Version 1)

Description

This is a testing construct for the hybrid promoter Lux and cI. In the presence of AI-1 LuxR binds to the promoter region inducing transcription. Thus, the output is GFP.

Notes

The OR repressor site may not be strong enough to out-compete the inducer.

Source

Preorder oligos and the registry.

Components

Access	Instance	Definition
public	BBa_K091107	BBa_K091107
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K091107	1,57	BBa_K091107
BBa_B0032	66,78	BBa_B0032
BBa_E0040	85,804	GFP
BBa_B0010	813,892	BBa_B0010
BBa_B0012	901,941	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091165/1

Member of these Collections

• iGEM Parts Registry