Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Heil, Danielle Nash, Shira Davis
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:40
Convergent Promoter System: Forward Module: rTT+lambda pR
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_K093003_sequence (Version 1) |
Description
This is the forward promoter for a convergent promoter system. It is the promoter BBa_R0051 that is repressible by BBa_c0051, with an upstream reverse TT to terminate anti-sense transcripts from its corresponding reverse promoter. The corresponding part for the convergent promoter system is BBa_K093004.Notes
We had to consider what was the forward and reverse efficiencies of the available TT sequences and whether it was worth it to synthesize a reverse TT. We synthesized one when we decided to synthesize the revers parts together with their closest adjacent parts. To make our convergent promoter system available for others who wish to have very tight repression of gene expression.Source
Synthetic, biobrick registry parts put together. The reverse compliment of BBa_B0015 is the reverse TT part. The promoter part is BBa_R0051.| Access | Instance | Definition |
| public public | BBa_K093011 BBa_R0051 | BBa_K093011 cI lam |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K093011 BBa_R0051 | 1,129 138,186 | BBa_K093011 cI lam |