Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ellen Martin
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:41
Construct with LacI/LAMBDAcI promoter, RBS, GFP, Terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K101005_sequence (Version 1) |
Description
Has a promoter which is dually repressed by LacI and LAMBDAcI. The RBS is the ribosomal binding site. The GFP is a green fluorescent protein, which is a reporter gene. The Terminator is used to terminate RNA polymerase's transcription.Notes
Majority of design considerations were dealt with during the design of the dually repressed promoter (K101001). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.Source
This construct is composed of: LacI promoter ( R0010), LAMBDAcI promoter (R0051), RBS (B0032), GFP (E0040), and Terminator (B1006).| Access | Instance | Definition |
| public public public public | BBa_K101001 BBa_B0032 BBa_E0040 BBa_B1006 | BBa_K101001 BBa_B0032 GFP BBa_B1006 |