BBa_K101016

BBa_K101016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K101016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yiannis Kaznessis

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:08:42

Dually repressed promoter with sites for TetR and P22MNT binding

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K101016_sequence (Version 1)

Description

Dual tetR/p22mnt promoter, RBS, GFP, terminator. This biobrick was constructed by digesting dual promoter tet/p22mnt with EcoRI and SpeI restriction enzymes, and ligating it to XbaI and PstI digested reporter (E0240), and Eco RI and PstI digested pSB3K3. It was used for the measurement of promoter strenghth.

Notes

This biobrick was constructed by digesting dual promoter tet/p22mnt with EcoRI and SpeI restriction enzymes, and ligating it to XbaI and PstI digested reporter (E0240), and Eco RI and PstI digested pSB3K3.

Source

BBa_K101000, B0032, E0240, B1006

Components

Access	Instance	Definition
public	BBa_K101000	BBa_K101000
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K101000	1,61	BBa_K101000
BBa_B0032	70,82	BBa_B0032
BBa_E0040	89,808	GFP
BBa_B0010	817,896	BBa_B0010
BBa_B0012	905,945	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K101016/1

Member of these Collections

• iGEM Parts Registry