Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karl Petersen
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:08:42
MioC Promoter (regulating tetR)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K101018_sequence (Version 1) |
Description
The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109).This promoter is upstream of a tetR (BBa_I15040), which is repressed by the binding of DNAa during initiation.
Notes
Site directed mutagenesis was used to create a Spe1 restriction site.Source
The MioC promoter is from K-12 and the tetR is the part BBa_I15040.| Access | Instance | Definition |
| public public | BBa_K101017 BBa_I15040 | MioC RBS.TetR |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101017 BBa_I15040 | 1,319 328,969 | MioC RBS.TetR |