Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kento Tominaga
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:42
Km-Blunt end
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1015013_sequence (Version 1) |
Description
This part changes cohesive end to blunt end on the prefix and suffix.Also this includes a gene which has resistance to kanamycin. Therefore it works as an antibiotic resistance marker to select colonies of E.coli which succeeded.
Notes
This part can insert other part easily between backbone.Source
pACYC177| Sequence Annotation | Location | Component / Role(s) |
| Pvu2 restriction site Nru1 restriction site Sma1 restriction site Km resistant gene Pvu2 restriction site Nru1 restriction site Sma1 restriction site | 2,7 9,14 15,20 125,937 1005,1010 998,1003 992,997 | feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |