Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maithili Krishnan
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:08:45
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1022121_sequence (Version 1) |
Description
This biobrick is one of the final constructs in Project Peptidor by Team TU Delft iGEM 2013 to check the sensing of AIP by E.coli and then production of the peptides followed by TetR production to activated the next set of the constructs.Notes
The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119Source
Clonned| Sequence Annotation | Location | Component / Role(s) |
| promoter BBa_K206000 AraI1 AraI2 BBa_I746103 BBa_I746100 B0034-sequence RBS agrC coding sequence BamHI site B0034-sequence RBS agrA coding sequence BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_I746104 BBa_K1022119 34 His 6 SUMO Signiferin 34 Tet R B0010 B0012 | 1,131 1,130 40,57 61,78 139,2299 139,2058 139,150 157,1404 1405,1410 1411,1422 1429,2058 2067,2146 2078,2121 2155,2195 2162,2181 2182,2195 2188,2188 2204,2299 1,2299 2300,2313 2314,2634 2635,2683 2688,2691 2705,3320 3395,3474 3483,3523 | promoter feature/promoter feature/BioBrick engineered_region feature/operator operator feature/operator operator engineered_region feature/BioBrick engineered_region feature/BioBrick feature/rbs ribosome_entry_site CDS feature/cds feature/misc sequence_feature feature/rbs ribosome_entry_site feature/cds CDS engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop polyA_site feature/polya feature/stop stop_codon engineered_region feature/BioBrick engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/tag tag sequence_feature feature/dna feature/rbs ribosome_entry_site CDS feature/cds feature/stop stop_codon feature/stop stop_codon |