Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
Constitutively Expressed dCas9 Operon
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1026000_sequence (Version 1) |
Description
This Constitutively Expressed dCas9 Operon is an assembly of the constitutive promoter BBa_J23100, a strong RBS BBa_B0034, coding sequence of dCas9 protein and a reliable terminator BBa_B0015.dCas9 is a mutant of Cas9, with its endonuclease activity wiped out. dCas9 acts as the sole protein in CRISPRi system (Qi et al., 2013).
Reference
QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.
Notes
The expression level of dCas9 shall be delicately controlled, so as to acquire continuous adjustment (regulation) of target genes.Source
The dCas9 comes from S. pyogenes.| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_B0034 BBa_K1026001 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K1026001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1026001 BBa_B0015 | 1,35 44,55 62,4174 4183,4311 | BBa_J23100 BBa_B0034 BBa_K1026001 BBa_B0015 |