Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hongyi WU
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
Constitutively Expressed gRNA targeting mRFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1026002_sequence (Version 1) |
Description
This Constitutively Expressed gRNA targeting mRFP is a no-BioBrick-scars assembly of the constitutive promoter BBa_J23100, DNA sequence of a gRNA that targets mRFP reporter and a reliable terminator BBa_B0015. This gRNA sequence comes from the following literature:QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.
Notes
gRNA shall be exactly transcribed out. Any extra nucleotides would potentially jeopardize the normal function of the gRNA. Therefore, BioBrick scars are not allowed here.Instead of cut-and-ligation, inverse PCR or In-Fusion is preferred for the assembly task.
In addition, a reliable terminator is applied here to ensure the termination of gRNA.
Source
The gRNA sequence is de novo synthesized.| Access | Instance | Definition |
| public public public | BBa_J23100 BBa_K1026003 BBa_B0015 | BBa_J23100 BBa_K1026003 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K1026003 BBa_B0015 | 1,35 36,137 138,266 | BBa_J23100 BBa_K1026003 BBa_B0015 |