Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jama Hagi-Yusuf
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:08:50
PhiC31 Intgrase and Recombination Directionality Factor Expression Cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1039014_sequence (Version 1) |
Description
A strong promoter and strong RBS (BBa_K608002) drive expression of PhiC31 integrase (BBa_K1039012) and PhiC31 recombination directionality factor (RDF) (BBa_K1039013). The RBS (BBa_B0034) is associated with RDF.Together, PhiC31 integrase and RDF (BBa_K1039012 and BBa_K1039013) catalyze site-specific recombination between PhiC31 attR and attL sites (Bba_K1039037 and Bba_K1039038).
They will also allow flipping of PhiC31 invertible promoter switches BBa_K1039011 and BBa_K1039010 from RL state to PB state.
Notes
The following mutation was made on PhiC31 integrase Base 51 is changed from T to C, silent mutation. The sequence was synthesized by a third party company.Source
Biobricks to make this part were synthesized by BioBasic Inc or from the iGEM 2013 distribution.| Access | Instance | Definition |
| public public public public | BBa_K608002 BBa_K1039012 BBa_B0034 BBa_K1039013 | BBa_K608002 BBa_K1039012 BBa_B0034 BBa_K1039013 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608002 BBa_K1039012 BBa_B0034 BBa_K1039013 | 1,55 62,2006 2015,2026 2033,2768 | BBa_K608002 BBa_K1039012 BBa_B0034 BBa_K1039013 |