Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ingmar Claes, Lukas Marcelis, Su Wang
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:08:58
TetR expression under glucose limiting conditions
| Types | DnaRegion |
| Roles | engineered_region Inverter |
| Sequences | BBa_K1060010_sequence (Version 1) |
Description
This device, which is in principle an invertor, produces the tetracyline repressor (TetR) (BBa_C0040) under glucose limiting conditions using the pCaiF promoter (BBa_K398326). The TetR used has an extra LVA tag, resulting in faster degredation and so quicker responses to changes in glucose concentrations. This device was used as an invertor to shut off methyl salicylate production (BBa_K1060003) in the absence of honeydew produced by aphids.Notes
no EcoRI, XbaI, NotI, SpeI or PstI sites presentSource
iGEM| Access | Instance | Definition |
| public public public public | BBa_K398326 BBa_B0032 BBa_C0040 BBa_K823017 | pCaiF BBa_B0032 tetR BBa_K823017 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K398326 BBa_B0032 BBa_C0040 BBa_K823017 | 1,51 60,72 79,763 772,866 | pCaiF BBa_B0032 tetR BBa_K823017 |