Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: mengyi sun
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:08:58
EF-1alpha-apoptin
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1061021_sequence (Version 1) |
Description
This device is a generator that constitutively express apoptin, a protein that can speficially induce apoptosis in cancer cells but not in normal cells. So that it can work like a general safeguard device for the protection of gene therapy.Notes
Although it has been try in a broad spectrum of cells and prove to be safe to the normal cells, it still has not been tried in ALL normal cells. So before using it to treat certain kinds of cancer, every team need to ensure that it want hurt the specify normal cells involve in the project. And it is a protein contain 121 amino acid that may big enough to induce immune reaction(although in the experiment of mouse it has not been reported.)Source
We got the apoptin from Zhuang's lab,and cloned the EF-1alpha promoter from the plasmid provided by Lei Xiao.| Access | Instance | Definition |
| public public | BBa_K1061011 BBa_K1061001 | BBa_K1061011 BBa_K1061001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1061011 BBa_K1061001 | 1,537 544,909 | BBa_K1061011 BBa_K1061001 |