Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:02
Inducible ampicillin resistance cassette + LuxR expression cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1073029_sequence (Version 1) |
Description
In complex with the inducer 3-oxo-hexanoyl-HSL, the activator LuxR binds to the Lux promoter and enables expression. This construct provides all the parts required to express the gene behind the Lux promoter. Only the inducer 3-oxo-hexanoyl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.Notes
no considerations.Source
The biobricks for this construct were taken from the 2013 distribution kit.| Access | Instance | Definition |
| public public public | BBa_K1073012 BBa_B0015 BBa_K1073015 | BBa_K1073012 BBa_B0015 BBa_K1073015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1073012 BBa_B0015 BBa_K1073015 | 1,946 955,1083 1092,1934 | BBa_K1073012 BBa_B0015 BBa_K1073015 |