BBa_K1073030

BBa_K1073030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1073030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team Braunschweig 2013

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:02

Inducible ampicillin resistance cassette + LasR expression cassette

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1073030_sequence (Version 1)

Description

In complex with the inducer PAI-1 (N-(3-oxododecanoyl)-homoserine lactone), the activator LasR binds to the Las promoter and enables expression. This construct provides all the parts required to express the gene behind the Las promoter. Only the inducer N-(3-oxododecanoyl)-homoserine lactone is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.

Notes

no considerations.

Source

The parts for this construct were taken from the 2013 distribution kit.

Components

Access	Instance	Definition
public	BBa_K1073013	BBa_K1073013
public	BBa_B0015	BBa_B0015
public	BBa_K1073016	BBa_K1073016

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1073013	1,1017	BBa_K1073013
BBa_B0015	1026,1154	BBa_B0015
BBa_K1073016	1163,2005	BBa_K1073016

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1073030/1

Member of these Collections

• iGEM Parts Registry