Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:02
Inducible ampicillin resistance cassette + LasR expression cassette
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1073030_sequence (Version 1) |
Description
In complex with the inducer PAI-1 (N-(3-oxododecanoyl)-homoserine lactone), the activator LasR binds to the Las promoter and enables expression. This construct provides all the parts required to express the gene behind the Las promoter. Only the inducer N-(3-oxododecanoyl)-homoserine lactone is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.Notes
no considerations.Source
The parts for this construct were taken from the 2013 distribution kit.| Access | Instance | Definition |
| public public public | BBa_K1073013 BBa_B0015 BBa_K1073016 | BBa_K1073013 BBa_B0015 BBa_K1073016 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1073013 BBa_B0015 BBa_K1073016 | 1,1017 1026,1154 1163,2005 | BBa_K1073013 BBa_B0015 BBa_K1073016 |