Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claudio Tiecher
Date created: 2013-09-09 11:00:00
Date modified: 2016-02-10 11:31:42
RBS Start-codon Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1085015_sequence (Version 1) |
Description
This BioBrick contains the coding sequence for two parts of the spider silk proteins (SubunitE1 and SubunitE2) with a Bacillus Subtilis ribosome binding site (RBS) and a Strep-tag infront of it.The RBS is there to allow the ribosomes to bind and start to translate the DNA.
The Strep-tag, fused at the N terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
SubunitE1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites.
SubunitE2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.
Notes
The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.Source
The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).| Access | Instance | Definition |
| public public | BBa_K1085000 BBa_K1085002 | BBa_K1085000 BBa_K1085002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1085000 BBa_K1085002 | 1,268 277,486 | BBa_K1085000 BBa_K1085002 |