BBa_K1088007

BBa_K1088007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2013-08-19 11:00:00

Date modified: 2015-05-08 01:09:06

E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1088007_sequence (Version 1)

Description

This composite part consists of ? subparts:

A Lac promoter: BBa_R0010
A strong RBS: BBa_J15001
The Dxs protein from E. coli: BBa_K118000
A 10 aa protein linker: BBa_K105012
A GFP reporter protein: BBa_E0040

Notes

We used USER cloning to assemble this part and therefore there are no scar sites. However we did insert a scarsite between the RBS and the start codon aswell as between the promoter and RBS, for optimal expression of our protein.

Source

The subparts of this composite part is derived from E. coli K-12 MG1655. The GFP derives from Aequorea victoria.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K1088021	BBa_K1088021
public	BBa_J15001	BBa_J15001
public	BBa_K118000	dxs
public	BBa_K105012	BBa_K105012
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K1088021	201,206	BBa_K1088021
BBa_J15001	207,216	BBa_J15001
BBa_K118000	217,2082	dxs
BBa_K105012	2083,2112	BBa_K105012
BBa_E0040	2113,2832	GFP

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1088007/1

Member of these Collections

• iGEM Parts Registry