BBa_K1088008

BBa_K1088008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2013-08-19 11:00:00

Date modified: 2015-05-08 01:09:06

B. subtilis dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1088008_sequence (Version 1)

Description

This composite part consists of 5 parts

A lac promoter: BBa_R0010
A strong RBS: BBa_J15001
The Dxs from B. subtilis: BBa_K1088000
A 10 aa protein linker: BBa_K105012
A GFP reporter protein: BBa_E0040

Notes

The system was assembled with USER cloning but we inserted a scar site between the RBS and start codon aswell as between the promoter and the RBS. We did this to improbe translational efficiency. We used the terminator in the pSB1C3 plasmid.

Source

The parts mainly derive from E. coli K-12 MG1655 but the GFP as an exception comes from Aequorea victoria

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K1088021	BBa_K1088021
public	BBa_J15001	BBa_J15001
public	BBa_K1088000	dxs (B. su
public	BBa_K105012	BBa_K105012
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K1088021	201,206	BBa_K1088021
BBa_J15001	207,216	BBa_J15001
BBa_K1088000	217,2118	dxs (B. su
BBa_K105012	2119,2148	BBa_K105012
BBa_E0040	2149,2868	GFP

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088008/1

Member of these Collections

• iGEM Parts Registry