Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-08-19 11:00:00
Date modified: 2015-05-08 01:09:06
B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1088009_sequence (Version 1) |
Description
This is the same part as BBa_K1088008, with the exception of LacI under the control of a constitutive promoter to ensure regulation of the Lac PromoterThis part consists of 7 subparts:
A constitutive promoter: BBa_J23106
A strong RBS: BBa_B0030
The LacI repressor protein, regulating the lac promoter: BBa_C0012
An artificial terminator: BBa_B1002
and the rest is similar to BBa_K1088008
Notes
The terminator aswell as the RBS and promoter was part of the reverse primer and forward primer for the LacI part, respectively. However we did insert a scar sequence between the RBS and startcodon to improve efficiency. There is also scar sites between the terminator (BBa_10002) and the rest of this composite part (BBa_K1088008). In the BBa_K1088008 part there is specific sites for scars aswell. Go to that parts page to investigate further.Source
With the exception of the artificial terminator and the GFP part from BBa_K1088008 (GFP = BBa_E0040), everything derives from E. coli K-12 MG1655| Access | Instance | Definition |
| public public | BBa_K1088020 BBa_K1088008 | BBa_K1088020 BBa_K1088008 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1088020 BBa_K1088008 | 1,1218 1227,4094 | BBa_K1088020 BBa_K1088008 |