Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Rosendahl Andreassen
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:06
lacI:LVA device (constitutive promoter, RBS and terminator)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1088020_sequence (Version 1) |
Description
The trancsriptional repressor lacI with LVA tag for faster degradation (BBa_C0012)under the constitutivly active promoter (BBa_J23106), with a RBS (BBa_B0030) and with a terminator (BBa_B1002).This device can be used to overexpress lacI with the purpose of repressing transcription from high-copy plasmids containting the lac promoter (BBa_R0010). The repression can be relieved by allosteric binding of IPTG to lacI, thereby promoting transcription from the lac promoter.
Notes
Used a strong terminator so that the composite part can be used in front of the lac promoter without unwanted transcription of the regulated gene.Source
The source of the part will be its components.| Access | Instance | Definition |
| public public public public public | BBa_J23106 BBa_B0030 BBa_K1088022 BBa_K1088023 BBa_B1002 | BBa_J23106 BBa_B0030 BBa_K1088022 lacI-LVA BBa_B1002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_B0030 BBa_K1088022 BBa_K1088023 BBa_B1002 | 1,35 36,50 51,56 57,1184 1185,1218 | BBa_J23106 BBa_B0030 BBa_K1088022 lacI-LVA BBa_B1002 |