Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-08-27 11:00:00
Date modified: 2015-05-08 01:09:06
B. subtilis dxs (lac promoter without lac inhibitor)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1088011_sequence (Version 1) |
Description
This part has the B. sub dxs (BBa_K1088000) controlled by the common Lac promoter. Notice that there is no LacI in this system, which is required for regulation of the Lac promoter.Notes
We forgot to acknowledge the need for high expression of LacI to keep the regulation of the lac promoter vaild.The constructs where assembled with USER cloning, and therefore there are no scarsites between the parts. There are however inserted scarsites between the RBS and the coding region aswell as between the lac promoter and the RBS to improve translational efficiency
Source
The Lac promoter derives from E. coli. The Dxs protein is from B. sub| Access | Instance | Definition |
| public public public public | BBa_R0010 BBa_K1088021 BBa_J15001 BBa_K1088000 | LacI BBa_K1088021 BBa_J15001 dxs (B. su |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_K1088021 BBa_J15001 BBa_K1088000 | 1,200 201,206 207,216 217,2118 | LacI BBa_K1088021 BBa_J15001 dxs (B. su |