BBa_K1088012

BBa_K1088012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2013-08-27 11:00:00

Date modified: 2015-05-08 01:09:06

E. coli dxs (lac promoter without lac inhibitor)

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1088012_sequence (Version 1)

Description

This part has the E. coli dxs (BBa_K1088000) controlled by the common Lac promoter. Notice that there is no LacI in this system, which is required for regulation of the Lac promoter.

Notes

All parts are from E. coli

Source

We forgot to acknowledge the need for high expression of LacI to keep the regulation of the lac promoter vaild. The constructs where assembled with USER cloning, and therefore there are no scarsites between the parts. There are however inserted scarsites between the RBS and the coding region aswell as between the lac promoter and the RBS to improve translational efficiency

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K1088021	BBa_K1088021
public	BBa_J15001	BBa_J15001
public	BBa_K118000	dxs

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K1088021	201,206	BBa_K1088021
BBa_J15001	207,216	BBa_J15001
BBa_K118000	217,2082	dxs

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088012/1

Member of these Collections

• iGEM Parts Registry