AraC

BBa_K1088017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Rosendahl Andreassen

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:06

AraC device (constitutive promoter, RBS and terminator)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1088017_sequence (Version 1)

Description

Coding region for the LacI protein derived from E. coli. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG (Isopropyl β-D-1-thiogalactopyranoside) binds to LacI and inhibits its operation, thereby promoting transcription.

Notes

The GTG start codon was changed into ATG

Source

>gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655

Components

Access	Instance	Definition
public	BBa_J23106	BBa_J23106
public	BBa_B0030	BBa_B0030
public	BBa_K1088022	BBa_K1088022
public	BBa_K1088005	araC
public	BBa_B1002	BBa_B1002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23106	1,35	BBa_J23106
BBa_B0030	36,50	BBa_B0030
BBa_K1088022	51,56	BBa_K1088022
BBa_K1088005	57,935	araC
BBa_B1002	936,969	BBa_B1002

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088017/1

Member of these Collections

• iGEM Parts Registry