BBa_K1088019

BBa_K1088019 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088019

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Rosendahl Andreassen

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:06

LacI device (constitutive promoter, RBS and terminator)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1088019_sequence (Version 1)

Description

The trancsriptional repressor lacI (BBa_K1088018)under the constitutivly active promoter (BBa_J23106), with a RBS (BBa_B0030) and with a terminator (BBa_B1002).

This device can be used to overexpress lacI with the purpose of repressing transcription from high-copy plasmids containting the lac promoter (BBa_R0010). The repression can be relieved by allosteric binding of IPTG to lacI, thereby promoting transcription from the lac promoter.

Notes

Used a strong terminator so that the composite part can be used in front of the lac promoter without unwanted transcription of the regulated gene.

Source

The source of the part will be its components.

Components

Access	Instance	Definition
public	BBa_J23106	BBa_J23106
public	BBa_B0030	BBa_B0030
public	BBa_K1088022	BBa_K1088022
public	BBa_K1088018	lacI
public	BBa_B1002	BBa_B1002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23106	1,35	BBa_J23106
BBa_B0030	36,50	BBa_B0030
BBa_K1088022	51,56	BBa_K1088022
BBa_K1088018	57,1139	lacI
BBa_B1002	1140,1173	BBa_B1002

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088019/1

Member of these Collections

• iGEM Parts Registry