Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Rosendahl Andreassen
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:09:06
Dxs from B. subtilis with lac promoter and lacI (IPTG inducible)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1088027_sequence (Version 1) |
Description
The part consist of the dxs gene derived from B. subtilis under the control of the lac promoter and has a strong RBS.To repress expression from the lac promoter, the lacI gene under a constitutive promoter, with a strong RBS and a efficient terminator is placed counter-clockwise to the reporter fusion. The repression can then be relieved with addition of IPTG, which binds and inhibits the function of LacI.
The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.
Notes
The lac promoter was used to enable control of the expression, since overexpression of the dxs gene has proved to lower the growth rate.Source
The components specify the source| Access | Instance | Definition |
| public public | BBa_K1088019 BBa_K1088011 | BBa_K1088019 BBa_K1088011 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1088019 BBa_K1088011 | 1,1173 1182,3299 | BBa_K1088019 BBa_K1088011 |