Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Belen Cespedes
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:11
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1113501_sequence (Version 1) |
Description
This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (BBa_K1113701) which deliver into the carboxysome and the ssrA degradation tag (BBa_M0052) which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will ???protect??? the fluorescens, in any other case this will be degraded by the proteaseNotes
This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vectorSource
The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley). All the rest are bricks available in the Registry| Access | Instance | Definition |
| public public public public public public | BBa_I0500 BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I746916 BBa_M0052 | pBad/araC BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I746916 BBa_M0052 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I0500 BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I746916 BBa_M0052 | 1,1210 1219,1230 1237,2655 2664,2693 2700,3419 3428,3460 | pBad/araC BBa_B0034 BBa_K1113701 BBa_K105012 BBa_I746916 BBa_M0052 |