Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12
This is a MoClo level 0 destination vector.
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1114400_sequence (Version 1) |
Description
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site B on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites A on the 5' side and site B on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Source
iGEM kit| Sequence Annotation | Location | Component / Role(s) |
| BsaI BsaI SpeI SpeI SpeI MoClo Fusion Site A MoClo Fusion Site B alpha fragment of lacZ BbsI BbsI | 14,19 528,533 1,6 7,12 535,540 21,24 523,526 33,514 27,32 515,520 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds feature/misc sequence_feature sequence_feature feature/misc |