BBa_K1114510

BBa_K1114510 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114510
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:12

Level 1 MoClo device that expresses YFP under pTetR



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1114510_sequence (Version 1)

Description


pR40B2Y_FG


This is a Level 1 MoClo device that expresses YFP under the repressible promoter pTetR. It contains the Level 0 parts R0040_FB, BCD2_BC, E0030_CD, and B0015_DG within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.



Notes


pR40B2Y_FG


This is a Level 1 MoClo device that expresses YFP under the repressible promoter pTetR. It contains the Level 0 parts R0040_FB, BCD2_BC, E0030_CD, and B0015_DG within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.



Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits

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BBa_K1114510/1