Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Simin Jiang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:21
An L-Arabinose controlled endolysin from bacteriophage Φ
KZ
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1136005_sequence (Version 1) |
Description
L-arabinose induced KZ144 endolysin is a functional construct which contains the gene that encodes for the endolysin protein from the bacteriophage ΦKZ (BBa_K1136002) which targets Pseudomonas aeruginosa. The construct is under the control of an L-arabinose promoter (BBa_K206000) and the rrnb terminator (BBa_B0010). This part can be expressed in E. coli using 20% L-Arabinose.Notes
The endolysin gene was synthesized by GenScript to optimize codons and avoid illegal sites. The promotor and terminator were chosen for controllable expression, because the endolysin may be toxic in high copy number.Source
The endolysin gene is from bacteriophage ΦKZ. An E. coli promoter and terminator were used.| Access | Instance | Definition |
| public public public | BBa_K206000 BBa_K1136002 BBa_B0010 | pBAD BBa_K1136002 BBa_B0010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_K1136002 BBa_B0010 | 1,130 137,916 925,1004 | pBAD BBa_K1136002 BBa_B0010 |