Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachit Jain
Date created: 2013-09-05 11:00:00
Date modified: 2015-05-08 01:09:21
pAW50-linker-mcherry(-RBS) (ugaiGEM11)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K1138001_sequence (Version 1) |
Description
This plasmid consists of the mcherry gene (encoding red fluorescence protein) inserted in the backbone of pAW50 vector. EcoRI-NotI-XbaI-NsiI restriction sites have been introduced followed by a linker sequence followed by the mcherry sequence. The use of these sites will allow users to introduce any desired gene of their choice to be fused with the linker which is already fused with the mcherry sequence. This way any protein can be qualitatively studied in Methanogens via red fluorescence. Note:- the users will have to introduce the RBS sequence and start codon, delete the terminator sequence of their gene while cloning.Notes
Primer were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker.Source
mcherry was obtained from Discosoma sp.| Sequence Annotation | Location | Component / Role(s) |
| promoter EcoRI NotI XbaI NsiI linker sequence mcherry puramycin ampicillin | 18,204 205,210 211,218 220,225 227,232 233,260 261,968 1048,1647 2430,3290 | feature/promoter promoter sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/protein CDS sequence_feature feature/misc sequence_feature feature/misc |