BBa_K1141000

BBa_K1141000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1141000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Adrien Rapeaux

Date created: 2013-09-22 11:00:00

Date modified: 2015-05-08 01:09:23

Plac-RBS-mCherry-double terminator (IPTG-inducible)

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1141000_sequence (Version 1)

Description

This part contains all the necessary DNA to produce the red fluorescent protein mCherry with the PLac promoter. PLac repression is low in most E. coli strains and the promoter will be leaky. Efficient repression can be implemented by additionally transforming a strain with the pREP4 plasmid from the M15 E. coli strain of QIAGEN, or transforming the part into this strain.

Notes

None

Source

This part is an assembly of different biobricks available in the registry: BBa_R0010 and BBa_J06702.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_J06702	mCherry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_J06702	209,1077	mCherry

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1141000/1

Member of these Collections

• iGEM Parts Registry