BBa_K1159008

BBa_K1159008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1159008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rosario CICCONE
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:30

Secretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-Part



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1159008_sequence (Version 1)

Description

This part encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This part is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Notes

x

Source

Synthetic

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1159008/1