BBa_K1159008

BBa_K1159008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1159008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Rosario CICCONE

Date created: 2013-09-14 11:00:00

Date modified: 2015-05-08 01:09:30

Secretory Catechol-2,3-dioxygenase (IgKappa-SigP_XylE) in RFC[25] N-Part

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1159008_sequence (Version 1)

Description

This part encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This part is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Notes

x

Source

Synthetic

Components

Access	Instance	Definition
public	BBa_K1159304	BBa_K1159304
public	BBa_K1159993	BBa_K1159993
public	BBa_K648011	BBa_K648011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1159304	1,67	BBa_K1159304
BBa_K1159993	68,73	BBa_K1159993
BBa_K648011	74,991	BBa_K648011

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1159008/1

Member of these Collections

• iGEM Parts Registry