Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:31
Membrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unit
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1159212_sequence (Version 1) |
Description
This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyCatcher and a secretory SpyTag_nLuc (IgKappa-SigP_SpyTag_nLuc) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal SpyTag and C-terminal NanoLuc Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public public public | BBa_K1159206 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159208 | BBa_K1159206 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159208 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159206 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159208 | 1,1447 1448,1464 1465,2091 2092,2097 2098,2725 | BBa_K1159206 BBa_K1159996 BBa_K1159300 BBa_K1159995 BBa_K1159208 |