Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-08-07 11:00:00
Date modified: 2015-05-08 01:09:36
rbs+crtI (phytoene dehydrogenase)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K118005_sequence (Version 1) |
Description
This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.Notes
The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows:* crtImutf1: gtt tac agt aag gtt gcc
* crtImutf2: agt aac tct ctg ttt gtg c
In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
Source
rbs: Escherichia coli JM109 genomic DNA.crtI: Pantoea ananatis genomic DNA
| Access | Instance | Definition |
| public public | BBa_J15001 BBa_K118003 | BBa_J15001 BBa_K118003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15001 BBa_K118003 | 1,10 17,1498 | BBa_J15001 BBa_K118003 |