BBa_K118018

BBa_K118018 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K118018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-10-05 11:00:00
Date modified: 2015-05-08 01:09:37

rbs+glgC16 (glgC with G336D substitution)



    • Details

    Types
    DnaRegion

    Roles
    Translational_Unit

    engineered_region

    Sequences BBa_K118018_sequence (Version 1)

    Description

    This is the coding sequence of glgC (ADP-glucose pyrophosphorylase) from Escherichia coli JM109 with the substitution G336D, with added ribosome binding site (rbs). This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung et al., 1986; Meyer et al., 1988)

    Notes

    No special considerations

    Source

    Escherichia coli JM109 genomic DNA.

    Access Instance Definition
    public
    public
    		BBa_J15001
    BBa_K118016
    		BBa_J15001
    BBa_K118016

    Sequence Annotation Location Component / Role(s)
    BBa_J15001
    BBa_K118016
    		1,10
    17,1315
    		BBa_J15001
    BBa_K118016

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K118018/1
     

    Attachments

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